亚洲综合一区二区三区无码,野花社区日本在线观看免费观看3,亚洲小说区图片区另类春色,日韩人妻无码一区二区三区综合部

服務(wù)熱線

15021010459
產(chǎn)品中心
當(dāng)前位置:主頁 > 產(chǎn)品中心 > 相關(guān)檢測(cè)試劑 > ELISA檢測(cè)試劑盒 >Alpco 80-INSPO-E01 ALPCO 豬/狗胰島素ELISA檢測(cè)試劑盒

Alpco 80-INSPO-E01 ALPCO 豬/狗胰島素ELISA檢測(cè)試劑盒

簡(jiǎn)要描述:產(chǎn)品英文名稱:Porcine and Canine Insulin ELISA kit
產(chǎn)品中文名稱:豬/狗 胰島素ELISA檢測(cè)試劑盒
貨號(hào):80-INSPO-E01
廠家:美國(guó)Alpco公司
品牌:Alpco
規(guī)格:96T/盒
產(chǎn)品應(yīng)用:豬/狗胰島素蛋白ELISA檢測(cè)
保存溫度: 2-8℃
貨期:2~5天
詳情請(qǐng)。

  • 產(chǎn)品型號(hào):80-INSPO-E01
  • 廠商性質(zhì):代理商
  • 更新時(shí)間:2016-12-28
  • 訪  問  量:1393
詳細(xì)介紹
品牌ALPCO

產(chǎn)品英文名稱:Porcine and Canine Insulin ELISA kit
產(chǎn)品中文名稱:豬/狗 胰島素ELISA檢測(cè)試劑盒

§ 檢測(cè)原理:ALPCO胰島素(豬/狗)ELISA是一個(gè)夾層式免疫檢測(cè)試劑盒。96孔微孔板涂有一層固相胰島素特異性鼠單克隆抗體。將標(biāo)準(zhǔn)物,對(duì)照品和樣品一并添加到微型板小孔中與山葵過氧化物酶標(biāo)記的單克隆抗體結(jié)合(配體物),胰島素分子被夾在固相和配體物之間。然后將微孔板放在搖床上室溫溫育2小時(shí),700-900轉(zhuǎn)/分鐘。*次溫育結(jié)束,用清洗緩沖液清洗小孔,去除未結(jié)合配體。添加TMB作用底物,放在微孔板搖床上第二次溫育15分鐘,700-900轉(zhuǎn)/分鐘。第二次溫育期間TMB和配體結(jié)結(jié)合物反應(yīng)呈藍(lán)色。第二次溫育結(jié)束,添加反應(yīng)終止液,調(diào)節(jié)分光光度計(jì)到450nm測(cè)定OD值。由于發(fā)光強(qiáng)度與樣品中胰島素含量成比例,因此可以根據(jù)OD值確定胰島素含量。

§ 檢測(cè)過程:各添加25μl標(biāo)準(zhǔn)物,對(duì)照物和樣品→加75µl配體物→微孔板搖床溫育2小時(shí),700-900rpm→清洗緩沖液清洗6次→加100µl TMB作用物→微孔板搖床溫育15分鐘,700-900rpm→添加100µl反應(yīng)終止液→分光光度計(jì)450nm讀數(shù)

ALPCO Porcine and Canine Insulin ELISA kit英文簡(jiǎn)述:
The ALPCO Porcine and Canine Insulin ELISA kit is for the quantification of porcine or canine insulin concentrations in serum and plasma samples. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Porcine and Canine Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time. Additional information regarding the Porcine and Canine Insulin ELISA kit can be accessed by clicking on the “Protocol" link or “Support" tab above.

INTENDED USE
The ALPCO Insulin (Porcine/Canine) ELISA is for the quantitative determination of insulin in Porcine/ Canine serum and plasma.
PRINCIPLE OF THE ASSAY
The ALPCO Insulin (Porcine/Canine) ELISA is a sandwich type immunoassay. Mouse monoclonal antibodies specific for insulin are immobilized to the 96-well microplate as the solid phase. Standards, controls, and samples are added to the appropriate wells with a horseradish peroxidase enzyme labeled monoclonal antibody (Conjugate), resulting in insulin molecules being sandwiched between the solid phase and the Conjugate. After incubation on a microplate shaker at room temperature, the microplate wells are washed with Wash Buffer to remove unbound Conjugate. TMB Substrate is added to each well, and the microplate is again incubated on a microplate shaker at room temperature. During the second incubation, a blue color results from TMB Substrate reacting with bound Conjugate in the wells. Stop Solution is added and this stops the reaction and changes the color from blue to yellow. The optical density (OD) is measured by microplate reader at 450 nm with a reference wavelength of 620 nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample.

相關(guān)產(chǎn)品:

 

產(chǎn)品名稱
貨號(hào)樣本容量規(guī)格
Insulin ELISA80-INSHU-E0125µl96T/
Insulin Ultrasensitive ELISA80-INSHUU-E0125µl96T/
Insulin (Synthetic) ELISA80-SINHU-E0125µl96T/
Insulin (Mouse) ELISA80-INSMS-E0110µl96T/
Insulin (Mouse) High Range ELISA80-INSMSH-E015µl96T/
Insulin (Mouse) Ultrasensitive ELISA(5µl)80-INSMSU-E015µl96T/
Insulin (Mouse) Ultrasensitive ELISA(25µl)80-INSMSU-E0125µl96T/
Insulin (Rat) ELISA80-INSRT-E0110µl96T/
Insulin (Rat) High Range ELISA80-INSRTH-E015µl96T/
Insulin (Rat) Ultrasensitive ELISA(5µl)80-INSRTU-E015µl96T/
Insulin (Rat) Ultrasensitive ELISA(25µl)80-INSRTU-E0125µl96T/
Insulin (Bovine) ELISA80-INSBO-E0125µl96T/
Insulin (Ovine) ELISA80-INSOV-E0125µl96T/
Insulin (Porcine/Canine) ELISA80-INSPO-E0125µl96T/
C-Peptide ELISA80-CPTHU-E01.125µl96T/
C-Peptide (Mouse) ELISA80-CPTMS-E0110µl96T/
C-Peptide (Rat) ELISA80-CPTRT-E0110µl96T/
Proinsulin (Mouse) ELISA80-PINMS-E0110µl96T/
Proinsulin ELISA80-PINHU-E0150µl96T/
Proinsulin (Rat) ELISA80-PINRT-E0110µl96T/

重慶市華雅干細(xì)胞技術(shù)有限公司

 


產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
推薦產(chǎn)品

2025 版權(quán)所有 © 重慶市華雅干細(xì)胞技術(shù)有限公司  備案號(hào):渝ICP備14000349號(hào)-4 sitemap.xml 管理登陸 技術(shù)支持:化工儀器網(wǎng)

地址:重慶市江北區(qū)金渝大道153號(hào)8棟20-14 傳真:023-63419626 郵件:sales@ys-bio.com

重慶市華雅干細(xì)胞技術(shù)有限公司主要經(jīng)營(yíng)干細(xì)胞研究 干細(xì)胞治療產(chǎn)品 生物試劑 實(shí)驗(yàn)耗材 藥物研發(fā)等產(chǎn)品。

關(guān)注我們

服務(wù)熱線

400-021-2200

掃一掃,關(guān)注我們

又黄又肉的叫床文| 人妻少妇看a偷人无码| 亚洲色在线无码国产精品不卡| 亚洲av中文无码乱人伦在线观看| 日本人妻伦在线中文字幕| 中文无码av一区二区三区| 国产日韩欧美一区二区三区| 永久免费av无码网站性色av| 中文字幕一区二区三区四区五区| 欧美老熟妇乱大交xxxxx| 啦啦啦中文免费观看在线| 国产av网站| 99亚洲精品自拍av成人软件| 和少妇人妻邻居做爰无码| 人妻无奈被迫屈辱1-9| 亚洲A∨精品一区二区三区下载| 久久婷婷久久一区二区三区| 亚洲日本无码一区二区三区四区卡| 久久精品国产亚洲av忘忧草蜜臀| 色欲av综合av在线av| 99久久人妻精品免费二区| 最近中文字幕在线看免费完整版| 国产自拍av| 久久久久无码中| 日日噜噜夜夜狠狠久久人人| 国产偷国产偷亚洲高清app| 朋友销魂的人妻| 日韩人妻精品一区二区三区视频| 99精品欧美一区二区三区黑人| 精品人妻无码一区二区三区手机版| 亚洲av午夜国产精品无码中文字| 久久久久99精品国产片| 国产亚洲精久久久久久无码| 欧美乱大交XXXXX潮喷| 国99精品无码一区二区三区| 久久久久人妻一区二区三区| 天天躁日日躁狠狠躁| 日韩精品无码熟人妻视频| 无码一区二区三区视频| 一本大道久久东京热无码av| 国产精品日本无码久久一老a|